In-situ monitoring of xenobiotics using genetically engineered whole-cell-based microbial biosensors: recent advances and outlook

نویسندگان

چکیده

Industrialisation, directly or indirectly, exposes humans to various xenobiotics. The increased magnitude of chemical pesticides and toxic heavy metals in the environment, as well their intrusion into food chain, seriously threatens human health. Therefore, surveillance xenobiotics is crucial for social safety security. Online investigation by traditional methods not sufficient detection identification such compounds because high costs complexity. Advancement field genetic engineering provides a potential opportunity use genetically modified microorganisms. In this regard, whole-cell-based microbial biosensors (WCBMB) represent an essential tool that couples engineered organisms with operator/promoter derived from metal-resistant operon combined regulatory protein gene circuit. plasmid controls expression reporter gene, gfp, luc, lux lacZ, inducible promoter has been widely applied assay toxicity bioavailability. This review summarises recent trends development application mobile genes biomedical environmental concerns.

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ژورنال

عنوان ژورنال: World Journal of Microbiology & Biotechnology

سال: 2021

ISSN: ['0959-3993', '1573-0972']

DOI: https://doi.org/10.1007/s11274-021-03024-3